Journal: Genes to cells : devoted to molecular & cellular mechanisms

Article Title: PRMT4/CARM1 Is a Novel Factor Promoting DNA Double-Strand Break Repair.

doi: 10.1111/gtc.70031

Figure Lengend Snippet: FIGURE 3 | Catalytic activity of PRMT4 was indispensable for releasing PRMT4 from the DSB sites. (A) U2OS cells expressing GFP-PRMT4 (wild-type or R168A mutant) were subjected to laser micro-irradiation and live-cell imaging. The track of laser micro-irradiation was indicated by white triangles. Scale bar: 10 μm. (B) Relative GFP signal intensity at DSB sites in (A) was quantified. The signal intensity 1 min after laser micro- irradiation when the accumulation of PRMT4 reached maximum was set to 1 and plotted (mean ± SEM, n = 10). *p < 0.05.

Article Snippet: Soluble fraction was subjected to immunoprecipitation with an anti- GFP antibody coupled with magnetic beads (GFP- Trap_MA, ChromoTek, PlaneggMartinsried, Germany) or anti- PRMT4 antibody (Cell Signaling Technology, 12495T, 1 μg/100 μg cell extract) by rotating overnight at 4°C.

Techniques: Activity Assay, Expressing, Mutagenesis, Irradiation, Live Cell Imaging

Journal: Genes to cells : devoted to molecular & cellular mechanisms

Article Title: PRMT4/CARM1 Is a Novel Factor Promoting DNA Double-Strand Break Repair.

doi: 10.1111/gtc.70031

Figure Lengend Snippet: FIGURE 4 | PRMT4 was a novel factor promoting DSB repair. (A) Genotyping of a PRMT4 KO cell line. The target site of gRNA and primers used for genotyping are indicated with the dashed red line and black arrows, respectively (upper). The result of the agarose gel electrophoresis was shown (lower). Amplification of β-actin gene was the loading control. (B) Cell extracts prepared from U2OS (wild type) and PRMT4 KO cell lines were ex- amined by immunoblotting with the indicated antibodies. (C) Neutral comet assay was carried out with U2OS (wild type) and PRMT4 KO cell line. Relative tail moments that were obtained by normalizing with the tail moment of the undamaged sample were plotted for damaged and repaired samples. Mean and standard error are indicated with the horizontal bars. ***p < 0.0001. (D) U2OS (wild type) and PRMT4 KO cell line were treated with 150 μg/mL phleomycin for 2 h. After washing out phleomycin, cells were further cultured for the indicated periods. Immunoblotting analysis with the indicated antibodies was carried out. H2AX and α-tubulin were loading controls for γH2AX and PRMT4, respectively.

Article Snippet: Soluble fraction was subjected to immunoprecipitation with an anti- GFP antibody coupled with magnetic beads (GFP- Trap_MA, ChromoTek, PlaneggMartinsried, Germany) or anti- PRMT4 antibody (Cell Signaling Technology, 12495T, 1 μg/100 μg cell extract) by rotating overnight at 4°C.

Techniques: Agarose Gel Electrophoresis, Amplification, Control, Western Blot, Neutral Comet Assay, Cell Culture

Journal: Scientific Reports

Article Title: Discovery of an ApoE4-targeted small-molecule SirT1 enhancer for the treatment of Alzheimer’s disease

doi: 10.1038/s41598-025-96131-2

Figure Lengend Snippet: Antibodies and dilutions used for immunoblots.

Article Snippet: PRMT4 , Cell Signaling , 4438 S , 0.7361.

Techniques: Western Blot